Modelling the movement of vesicles in cells

Detection of the arrival of important proteins at the cell surface is a problem of intense biological interest. Here we create an ideal model of the arrival of proteins at the cell membrane, as seen by a total internal reflection fluorescence microscope. 

Introduction

We explore the cell through the lens of a total internal reflection fluorescence (TIRF) microscope, in particular with a view to enable the automatic detection of events of interest occurring in sequences of images of live biological cells undergoing their regular metabolic processes. In order to benchmark and indeed enable the algorithms to be developed to do this image processing, ideal models of the events need to be derived, as even human experts cannot be certain or agree about the classification of regions of the TIRF imagery.